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KMID : 0984920090110020125
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Journal of Skin Barrier Research
2009 Volume.11 No. 2 p.125 ~ p.125
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Expression and functional role of Sox9 in keratinocyte differentiation
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Ge Shi
Sohn Kyung-Cheol
Choi Dae-Kyoung
Lee Young
Kim Chang-Duk
Seo Young-Joon
Lee Jeung-Hoon
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Abstract
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Sox9 (SRY (sex determining region Y)-box 9) is an autosomal gene involved in the control of the male-determining pathway. It has been known that Sox9 is expressed in outer root sheath of hair follicle and exerts its action as an importance regulator in maintenance of stemness for hair cells. However, its expression and putative role in epidermal keratinocytes has not been well elucidated yet. In this study, we examined the expression and functional role of Sox9 in keratinocyte differentiation. Immunohistochemical staining showed that Sox9 is predominantly expressed in basal layer. Consistent with this result, Sox9 mRNA level was markedly decreased by the calcium treatment of cultured keratinocytes. And, Western blot also showed that the protein level for Sox9 was decreased by calcium in a temporal manner. To investigate its putative role, we made a recombinant adenovirus harboring the expression cassette for Sox9. Overexpression of Sox9 prevented calcium-induced loricin and involucrin expression. Consistent with, overexpression of Sox9 decreased the promoter activities of involucrin and loricrin. In addition, Sox9 overexpression resulted in significant down-regulation of the cell cycle-related genes p21. Interestingly, Sox9 expression was highly increased in several skin diseases such as psoriasis, BCC and SCC. Together, these results suggest that Sox9 is an important regulator for
keratinocyte proliferation and differentiation, and may have related with many of skin diseases that are characterized by the hyperproliferative potential of keratinocytes.
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KEYWORD
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Sox 9, Hair follicle, Keratinocyte differentiation
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